Effect of number of replicates on relative standard deviation. Each partial figure shows the effect of different numbers of combined replicates (abscissa) on the relative standard deviation of the population (ordinate). Values for five different settings of baseline/threshold (1–5; Table 1) are depicted. The green diamond marks the number of replicates with which a maximum of 15% relative standard deviation is achieved (for all settings). The titles state the name of the event and the nominal value of the measured reference material. Each partial figure is based on experimental data from a single 96-well plate. Dashed frames indicate experiments with plasmidic standard curves (plasmids were linearized before use).