Figure 2

Locations of asparagine repeats in P. falciparum gyrase genes and the structures of E. coli gyrases containing the corresponding regions. (A) The asparagine-repeat of Pf-GyrA is located in a region close to the region corresponding to the GyrA box of prokaryotic gyrases. This region often appears disordered in crystal structures. Two residues (Ser555 and Asp576) flanking the disordered region containing the GyrA box (560-QRRGGKG-566) are highlighted by magenta spheres (PDB code 1ZI0 [41] chain A). (B) The asparagine-repeat is encompassed in the Plasmodium specific insertion of gyrase B. The region corresponds to the ATP lid of Ec-GyrB, highlighted by magenta on the 24 kDa fragment structure (PDB code 1AJ6 [49]).