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Table 1 Summary of experiments previously carried out with P. falciparum gyrase proteins

From: Unique features of apicoplast DNA gyrases from Toxoplasma gondii and Plasmodium falciparum

Pf protein

Partner protein

Test

Result

References

GyrB (various constructs)

 

ATPase

Decreased ATP turnover rate compared to E. coliprotein

ATPase activity can be enhance

Dar et al., 2007 [35]; Ram et al., 2007 [34]; Dar et al., 2009 [37]

DNA binding

DNA binding activity through 45 amino acid insert region

Dar et al., 2009 [37]

Conformational change in response to nucleotide

Clamp closure can be modulated by nucleotide binding

Dar et al., 2009 [37]

Dimerization

Dimerization occurred in the absence of ATP

Dar et al., 2007 [35]

GyrB

Ec-GyrA (full length)

ATPase

Enhanced ATP turnover rate

Dar et al., 2007 [35]

Supercoiling

Lower supercoiling activity compared to E. coli gyrase

Dar et al., 2007 [35]; Dar et al., 2009 [37]

DNA cleavage

Introduced DNA breaks

Dar et al., 2007 [35]; Dar et al., 2009 [37]

GyrB

Pf-GyrA NTD

DNA cleavage

Introduced DNA breaks

Dar et al., 2007 [35]

Supercoiling

Failed in DNA supercoiling

Dar et al., 2007 [35]

GyrA NTD

Ec-GyrB (full length)

DNA cleavage

Introduced DNA breaks

Dar et al., 2007 [35]