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Table 1 Summary of experiments previously carried out with P. falciparum gyrase proteins

From: Unique features of apicoplast DNA gyrases from Toxoplasma gondii and Plasmodium falciparum

Pf protein Partner protein Test Result References
GyrB (various constructs)   ATPase Decreased ATP turnover rate compared to E. coliprotein
ATPase activity can be enhance
Dar et al., 2007 [35]; Ram et al., 2007 [34]; Dar et al., 2009 [37]
DNA binding DNA binding activity through 45 amino acid insert region Dar et al., 2009 [37]
Conformational change in response to nucleotide Clamp closure can be modulated by nucleotide binding Dar et al., 2009 [37]
Dimerization Dimerization occurred in the absence of ATP Dar et al., 2007 [35]
GyrB Ec-GyrA (full length) ATPase Enhanced ATP turnover rate Dar et al., 2007 [35]
Supercoiling Lower supercoiling activity compared to E. coli gyrase Dar et al., 2007 [35]; Dar et al., 2009 [37]
DNA cleavage Introduced DNA breaks Dar et al., 2007 [35]; Dar et al., 2009 [37]
GyrB Pf-GyrA NTD DNA cleavage Introduced DNA breaks Dar et al., 2007 [35]
Supercoiling Failed in DNA supercoiling Dar et al., 2007 [35]
GyrA NTD Ec-GyrB (full length) DNA cleavage Introduced DNA breaks Dar et al., 2007 [35]