From: Unique features of apicoplast DNA gyrases from Toxoplasma gondii and Plasmodium falciparum
Pf protein | Partner protein | Test | Result | References |
---|---|---|---|---|
GyrB (various constructs) | Â | ATPase | Decreased ATP turnover rate compared to E. coliprotein ATPase activity can be enhance | Dar et al., 2007 [35]; Ram et al., 2007 [34]; Dar et al., 2009 [37] |
DNA binding | DNA binding activity through 45 amino acid insert region | Dar et al., 2009 [37] | ||
Conformational change in response to nucleotide | Clamp closure can be modulated by nucleotide binding | Dar et al., 2009 [37] | ||
Dimerization | Dimerization occurred in the absence of ATP | Dar et al., 2007 [35] | ||
GyrB | Ec-GyrA (full length) | ATPase | Enhanced ATP turnover rate | Dar et al., 2007 [35] |
Supercoiling | Lower supercoiling activity compared to E. coli gyrase | |||
DNA cleavage | Introduced DNA breaks | |||
GyrB | Pf-GyrA NTD | DNA cleavage | Introduced DNA breaks | Dar et al., 2007 [35] |
Supercoiling | Failed in DNA supercoiling | Dar et al., 2007 [35] | ||
GyrA NTD | Ec-GyrB (full length) | DNA cleavage | Introduced DNA breaks | Dar et al., 2007 [35] |