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Figure 3 | BMC Bioinformatics

Figure 3

From: Exploring possible DNA structures in real-time polymerase kinetics using Pacific Biosciences sequencer data

Figure 3

Kinetics around CGG repeats. These three graphs show the average polymerase kinetics and their quantiles for the sequence GGCGGCGGCGG, found in 27 regions in the E. coli genome. The top graph shows the raw IPD values for the region, representing the time between base calls, in seconds. In the middle and bottom graphs, the 2 bp (4 bp) smoothing represents wavelet smoothing over a two (four) nucleotide region within the reads. The smoothed values can be thought of as similar to average polymerase kinetics over the 2 bp and 4 bp windows. For each measurement, the black vertical lines are the 90% quantile, the grey vertical lines are the 95% quantile. A total of 1217 reads were used in the analysis.

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