SFMetrics main window screenshot for orientation and examples of different functions. DNA molecules (pUC19) were used for obtaining the images analysed here. One DNA molecule was selected (white-blue square selection tool) for measuring the volume in the contiguous window where the pixels taken into account for volume calculation are surrounded by a red line after using ‘Plot line’ and defining ‘Line width’ (yellow lines). The graphs show the height and the FWHM, respectively, along the measured line. Skeleton length is shown in the right window.