Figure 4
From: SFMetrics: an analysis tool for scanning force microscopy images of biomolecules
Example analysing multiple features of DNA-protein complexes with SFMetrics. A) SFM image of filaments formed by human RAD51 protein on double-stranded DNA. Image size: 2×2 μm, height is represented by color in the range of −2.5-2.5 nm. B) Volume analysis of the filament zoomed in panel A. The filament is surrounded by free protein complexes, not bound to DNA. However, these do not affect volume calculations performed only for the biggest object (the filament). i.u. = intensity units. C) Skeleton length tool is used for estimating the length of the filament. After appropriately adjusting the threshold slider only the filament axis is highlighted and background is discarded. Angle tool can be used to determine kink’s openness (in red).