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Figure 6 | BMC Bioinformatics

Figure 6

From: SFMetrics: an analysis tool for scanning force microscopy images of biomolecules

Figure 6

Example of high-throughput analysis of protein complexes. A) Screenshot of SFMedges (obtained by clicking on SFMedges button shown in Figure 2) showing the analysis of RAD50-MRE11 complexes deposited on mica. Selected complexes for the analysis are delimited by a red line after the user has determined the selection stringency with ‘Adjust threshold’. This results in a histogram showing the volume distribution of the selected complexes, displayed at the right. The data has been normalized to kiloDaltons (kDa) using the volume of E.coli RNA polymerase (450 kD) determined separately as a standard. The histogram here has been limited to display particles larger than 50 kDa and smaller than 2000 kDa, and the number of bins set at 15. B) Zoom of the image in panel A after detecting the edges of the complexes (zoom size: 390x390 nm). C) Comparison with other threshold methods. Same zoomed area as in panel B analysed by direct intensity thresholding discarding pixels with values below 10%, 15%, and 20% of the maximum image intensity as conventionally applied.

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