Figure 2

Specificity comparison of ViVaMBC with LoFreq, V-phaser 2 and ShoRAH. The frequencies of all minor variants discovered in the three mixtures 1:200, 1:100 and 1:50 are plotted for ViVaMBC, LoFreq, V-phaser 2 and ShoRAH. Note that these variants are at the codon level for ViVaMBC and at the SNP level for the other methods. The false positive variants are indicated with black dots and the true positives with gray crosses. It is clear that although far more false-positive findings are discovered with ViVaMBC, the distinction with the true positives is more apparent.