Figure 4

Comparison of wavClusteR with PARalyzer on publicly available PAR-CLIP data. (A) Barplot representing overlapping or exclusive clusters identified by PARalyzer (with parameters n=1 (left) and n=2 (right)) and wavClusteR on AGO2 PAR-CLIP data. Note that the number of overlapping clusters called by either method differ as there is no one-to-one correspondence between them. (B) Distribution of RSF values for T→ C transitions localizing within clusters exclusively identified by PARalyzer (n=1) and wavClusteR (n=2). (C) Annotation of method-specific clusters to distinct features of the human transcriptome. Blue and orange circles denote clusters exclusively identified by wavClusteR and PARalyzer, respectively. (D) Normalized number of RNA-Seq reads from transcripts overlapping with clusters exclusively identified by by either method. (E) Seed enrichments over randomized background for microRNA seeds of microRNA expressed in HEK293 cells within clusters exclusively identified by PARalyzer and wavClusteR. (F) Same as E, but for the mir-124 seed in AGO2 PAR-CLIP from cells transfected with this microRNA.