A unified framework for estimating parameters of kinetic biological models

BMC Bioinformatics

Table 2 Final parameter estimation result with confidence intervals after solving the non-identifiability

Parameter name	Original value	Value	σ+	σ-
K_i1Fru	1.00	0.99	1.19	0.18
K_i2Glc	1.00	1.00	2.07	0.40
K_i3G6P	0.10	0.10	0.11	0.10
K_i6Suc6P	0.07	0.05	0.09	0.01
K_i6UDPGlc	1.40	1.16	2.32	0.05
K_m6UDP	0.30	0.40	0.63	0.18
K_m6Suc6P	0.10	0.16	0.56	0.06
V_max11	1.00	0.99	1.45	0.09
K_m11Suc	100.00	99.59	102.48	96.70
*K_i4F6P	10.00	10.00	-	-
*V_max6r	0.20	0.20	-	-
*K_i6F6P	0.40	0.40	-	-

*Parameters that were measured.
To achieve this, three non-identifiable parameters (K_i4F6P, V_max6r and K_i6F6P) were “explicitly” measured and the rest were estimated. During each successive estimation phase, parameters estimated with high confidence in a previous run are fixed. The asymmetric confidence interval has upper bound σ + and lower bound σ-.

ISSN: 1471-2105