Figure 2
From: Fully automated pipeline for detection of sex linked genes using RNA-Seq data
Simplified workflow. Mother’s reads are assembled with Trinity assembler and newly created contigs are then used as a reference to which all reads are mapped. This yields into 4 bam files storing alignments. After applying filters, X-linked contigs are identified. For detection of Y-linked genes, reference contigs are assembled from male reads (together male parent and male progeny)