Experimental laboratory verification of sex-linked contigs. A) PCR products of candidate X-linked contigs were sequenced and clustered. Father and daughter variants cluster together, which confirms X-linkage of a selected gene. Note that SNP marked in blue color is shared only among sequences of father and daughters. Another SNP in yellow represents sequencing error. B) PCR products of candidate Y-linked contigs. For every contig/gene genomic DNA of 7 individuals (4 males, 3 females) was used as a template: father (Reckovice, CZ), brother of father (Reckovice, CZ), two sisters of father (Reckovice, CZ), mother (Almería, Spain), two brothers of mother (Almería, Spain). Product is present only in male individuals.