Figure 5From: Fully automated pipeline for detection of sex linked genes using RNA-Seq data Experimental laboratory verification of sex-linked contigs. A) PCR products of candidate X-linked contigs were sequenced and clustered. Father and daughter variants cluster together, which confirms X-linkage of a selected gene. Note that SNP marked in blue color is shared only among sequences of father and daughters. Another SNP in yellow represents sequencing error. B) PCR products of candidate Y-linked contigs. For every contig/gene genomic DNA of 7 individuals (4 males, 3 females) was used as a template: father (Reckovice, CZ), brother of father (Reckovice, CZ), two sisters of father (Reckovice, CZ), mother (Almería, Spain), two brothers of mother (Almería, Spain). Product is present only in male individuals.Back to article page