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Table 2 List of filtering rules used for identification of sex-linked contigs; filtering rules for identification of Y-linked genes

From: Fully automated pipeline for detection of sex linked genes using RNA-Seq data

Minimal and maximal number of reads.

Each male must have at least 15 reads mapped, each female can have at most 3 reads mapped.

Fraction of female and male reads.

female reads/(female reads + male reads) < 0.03.

Male reads must be more abundant than female reads.

mother reads < father reads;

mother reads < son reads;

daughter reads < father reads;

daughter reads < son reads