The importance of a good barcode design. This image shows 2 barcodes, completed with the restriction enzyme recognition site of ApeKI. If these barcodes are used for the demultiplexing of GBS or RAD data with the ApeKI enzyme, the software will recognize the correct barcodes (sample) because of the Hamming distance between the barcodes. When the barcodes are used with another or no enzyme, the barcodes will have a different distance. This could result in misdemultiplexing of the reads.