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Table 1 Demultiplexing statistics as an accumulation of ten synthetic data sets, totaling 12,579,549 synthetic reads

From: GBSX: a toolkit for experimental design and demultiplexing genotyping by sequencing experiments

Paired end reads

RE

# Misassigned

Misassignments

# Correctly

Sensitivity

 

Site

 

per million reads

demultiplexed

 

GBSX GBS

Y

7

0.6

12,394,916

98.53%

GBSX RAD

Y

7

0.6

12,394,916

98.53%

GBSX NA

N

54

4.3

12,410,223

98.65%

Stacks process_radtags

Y

0

0.0

7,756,281

61.66%

Stacks process_shortreads

N

0

0.0

10,529,102

83.70%

Sabre

N

54

4.3

12,410,223

98.65%

Single end reads

RE

# Misassigned

Misassignments

# Correctly

Sensitivity

 

Site

 

per million reads

demultiplexed

 

GBSX GBS

Y

7

0.6

12,394,916

98.53%

GBSX RAD

Y

7

0.6

12,394,916

98.53%

GBSX NA

N

54

4.3

12,410,223

98.65%

Stacks process_radtags

Y

0

0.0

7,820,903

62.17%

Stacks process_shortreads

N

0

0.0

10,530,753

83.71%

Sabre

N

54

4.3

12,410,223

98.65%

  1. NA indicates an enzyme was not provided to GBSX for demultiplexing. The RE Site column indicates if the reads did or did not contain the ApeKI restriction enzyme sequence.