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Table 2 Trimming statistics on demultiplexed reads from Table 1

From: GBSX: a toolkit for experimental design and demultiplexing genotyping by sequencing experiments

Paired end reads

RE

# Correctly

Sensitivity

Triming errors

 

Site

trimmed

 

per thousand reads

GBSX GBS

Y

12,381,497

99.89%

1

GBSX RAD

Y

12,313,669

99.34%

7

GBSX NA

N

12,364,365

99.63%

4

Stacks process_radtags

Y

7,407,377

95.50%

45

Stacks process_shortreads

N

7,190,394

68.29%

317

Single end reads

RE

# Correctly

Sensitivity

Triming errors

 

Site

trimmed

 

per thousand reads

GBSX GBS

Y

11,878,172

95.83%

42

GBSX RAD

Y

10,821,969

87.31%

127

GBSX NA

N

10,835,239

87.31%

127

Stacks process_radtags

Y

7,421,337

94.89%

51

Stacks process_shortreads

N

7,191,440

68.29%

317

  1. The RE Site column indicates if the reads did or did not contain the ApeKI restriction enzyme sequence. Sabre does not perform read trimming and is therefor not included in this comparison.