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Table 30 Top entropy hits in E. coli

From: Secondary structural entropy in RNA switch (Riboswitch) identification

E. coli

Start

End

Strand

Upstream operon

Dist. to upstream

MFE

MFE p. Val.

GC

RND

RND p. Val.

Uracil

Dist. to downstream

Downstream operon

Probability

100 nt

4083889

4083988

forward

yiiF

-5848

-38.4

0.0267

0.53

58.6367989

0.0365

0.29

102

fdhD

0.789

100 nt

187962

188061

forward

cdaR

-4293

-36.4

0.0466

0.53

59.0985985

0.0229

0.32

1702

rpsB,tff,tsf

0.776

100 nt

952485

952584

forward

ycaK

-2955

-36.8

0.0419

0.52

58.3203011

0.0494

0.27

3452

ycaP

0.765

100 nt

4115038

4115137

forward

uspD,yiiS

-3245

-37

0.0396

0.53

58.3563995

0.0477

0.33

1452

zapB

0.756

E. coli

Start

End

Strand

Upstream operon

Dist. to upstream

MFE

MFE p. Val.

GC

RND

RND p. Val.

Uracil

Dist. to downstream

Downstream operon

Probability

150 nt

2686923

2687072

forward

hmp

-1797

-56.00

-

0.5333

90.7522964

0.0077

0.32000

6822

mltF

0.8671584129

150 nt

452721

452870

forward

yajQ

-8244

-60.90

-

0.5200

88.2920990

-

0.23333

897

bolA

0.8664909005

150 nt

1100699

1100848

forward

ycdZ

-610

-58.70

-

0.4933

87.8781967

-

0.30666

2397

csgA,csgB,csgC

0.8559710383

150 nt

2887386

2887535

forward

iap

-11667

-56.40

-

0.5333

89.1240005

0.0294

0.31333

2772

queD

0.8254097700

150 nt

3467187

3467336

forward

gspO1

-2866

-56.10

-

0.5200

88.5419006

0.0450

0.29333

8397

slyX

0.8172816634

150 nt

2553118

2553267

forward

amiA,hemF

-893

-57.40

-

0.5133

87.6467972

-

0.28666

3597

intZ

0.8125300407

150 nt

2660264

2660413

forward

ryfA

-8005

-56.90

-

0.5000

87.1239014

-

0.32000

1122

suhB

0.8031908870

150 nt

1766798

1766947

forward

lpp

-11038

-57.00

-

0.4800

85.6548004

-

0.25333

222

ydiK

0.7757616639

150 nt

1718374

1718523

forward

slyB

72

-58.70

-

0.4867

85.1240005

-

0.32666

597

ydhI,ydhJ,ydhK

0.7731205821

150 nt

4356712

4356861

forward

yjdK,yjdO

-5529

-58.80

-

0.5200

86.0333023

-

0.26000

9897

fxsA

0.7661048174

150 nt

149580

149729

forward

yadD

-1631

-57.80

-

0.4600

84.3807983

-

0.30666

12447

hrpB

0.7651519775

150 nt

4604476

4604625

forward

yjjZ

-334

-57.20

-

0.4867

85.4507980

-

0.27333

1272

holD,rimI,yjjG

0.7621335387

150 nt

3120069

3120218

forward

C0719

-389

-56.40

-

0.5267

87.1032028

-

0.27333

6147

glcC

0.7610746622

150 nt

1982024

1982173

forward

uspC

-3740

-56.20

-

0.5333

87.3750000

-

0.26666

2847

ftnB

0.7596676350

150 nt

3921878

3922027

forward

cbrB,cbrC

-25167

-56.00

-

0.4933

85.5883026

-

0.30666

3222

asnA

0.7384917736

150 nt

790921

791070

forward

aroG

-4934

-57.00

-

0.5333

86.4469986

-

0.28000

2997

acrZ

0.7345629930

150 nt

4482291

4482440

forward

yjgN

-3263

-58.30

-

0.5200

85.4577026

-

0.28666

1872

lptF,lptG

0.7340587974

150 nt

518357

518506

forward

ybbL,ybbM

-1692

-57.10

-

0.5000

85.1729965

-

0.26000

522

ybbA,ybbP

0.7300664783

150 nt

1167546

1167695

forward

ycfJ

-106

-57.70

-

0.5333

85.9982986

-

0.27333

672

bhsA

0.7274026275

150 nt

1642496

1642645

forward

cspF

-2326

-56.00

-

0.5333

86.4957962

-

0.25333

1347

ydfV

0.7190257311

150 nt

3916103

3916252

forward

cbrB,cbrC

-19392

-60.90

-

0.5200

84.0416031

-

0.30000

8997

asnA

0.7181233764

150 nt

3258127

3258276

forward

yhaK,yhaL

-4819

-55.70

-

0.5267

86.1580963

-

0.26000

7197

tdcR

0.7085512877

150 nt

3721360

3721509

forward

insK

-1209

-57.70

-

0.5133

84.8648987

-

0.26000

2472

wecH

0.7070741653

150 nt

2438211

2438360

forward

flk

-1165

-58.50

-

0.5267

84.9561996

-

0.24000

1497

mnmC

0.7053987384

150 nt

1268246

1268395

forward

kdsA,ychA,ychQ

75

-56.90

-

0.4933

84.3839035

-

0.29333

222

rdlA

0.7012539506

150 nt

219458

219607

forward

arfB,nlpE,yaeQ

-3400

-59.90

-

0.5267

84.2009964

-

0.27333

3297

gmhB

0.6966923475

150 nt

3514668

3514817

forward

frlA,frlB,frlC,frlD,frlR

-11784

-56.50

-

0.5333

85.6490021

-

0.27333

6147

mrcA

0.6895118356

150 nt

3313884

3314033

forward

psrO

-4385

-55.60

-

0.5067

84.9284973

-

0.28666

2697

argG

0.6809250712

150 nt

649808

649957

forward

ybdR

-7180

-58.10

-

0.5333

84.6990967

-

0.25333

1572

dpiA,dpiB

0.6750817299

150 nt

2243989

2244138

forward

yeiG

-1142

-57.10

-

0.5333

84.3582993

-

0.26000

3672

yeiH

0.6381362677

150 nt

4253685

4253834

forward

ubiA,ubiC

-1695

-55.80

-

0.5267

84.4711990

-

0.29333

897

dgkA

0.6285824776

150 nt

2866503

2866652

forward

ygbN

-1937

-56.80

-

0.5333

84.2586975

-

0.30000

8022

iap

0.6268372536

150 nt

3576825

3576974

reverse

yhhW

-69

-55.60

-

0.4800

88.6371994

0.0419

0.30666

154

gntK,gntR,gntU

0.8547886610

150 nt

260750

260899

reverse

yafW,yafX,yafY,ykfB,ykfF,ykfG,ykfH,ykfI

-1723

-62.00

-

0.5267

86.8554001

-

0.32000

1504

phoE

0.8323625326

150 nt

2195866

2196015

reverse

yehS

-13803

-58.00

-

0.5333

88.6897964

0.0405

0.27333

3754

mrp

0.8320623040

150 nt

4176725

4176874

reverse

sroH

-11546

-58.80

-

0.5333

88.1481018

-

0.28000

3754

coaA

0.8252514601

150 nt

133211

133360

reverse

speD,speE,yacC

-1498

-56.40

-

0.5133

88.0559998

-

0.28000

2029

yacH

0.8126859665

150 nt

44332

44481

reverse

apaG,apaH,lptD,pdxA,rsmA,surA

-5969

-64.00

-

0.5333

85.3160019

-

0.26000

2479

caiA,caiB,caiC,caiD,caiE,caiT

0.8009238243

150 nt

2248916

2249065

reverse

nupX

-1922

-55.70

-

0.5333

88.3962021

-

0.28000

1354

yeiE

0.7910096645

150 nt

2774331

2774480

reverse

ypjA

-1758

-55.60

-

0.5200

87.4978027

-

0.26666

3229

ypjM_1,ypjM_2

0.7727379203

150 nt

2184269

2184418

reverse

yehA,yehB,yehC,yehD

-1054

-56.20

-

0.5333

87.6942978

-

0.30666

529

rcnR

0.7722578049

150 nt

1655707

1655856

reverse

mlc,ynfK

-8762

-58.20

-

0.5267

86.6440964

-

0.24000

304

ynfC

0.7716723680

150 nt

2168389

2168538

reverse

gatR_2

-951

-56.70

-

0.5133

86.7958984

-

0.31333

304

gatR_1

0.7715415359

150 nt

3481959

3482108

reverse

yhfA

-1398

-57.90

-

0.5333

86.7799988

-

0.25333

2854

kefB,kefG,yheV

0.7633723617

150 nt

314642

314791

reverse

ykgA

-953

-58.20

-

0.5067

85.1943970

-

0.32000

2254

ykgR

0.7403761148

150 nt

2783559

2783708

reverse

ileY

-146

-56.20

-

0.4667

84.4744034

-

0.26666

604

ypjC

0.7329583764

150 nt

4050403

4050552

reverse

glnA,glnG,glnL

-1410

-56.70

-

0.5267

86.2409973

-

0.27333

1729

yihA

0.7301918864

150 nt

2465055

2465204

reverse

yfdK,yfdL,yfdM,yfdN,yfdO

-3965

-58.50

-

0.5333

85.6035004

-

0.25333

2104

mlaA

0.7242872119

150 nt

2809717

2809866

reverse

luxS

-2444

-56.20

-

0.5333

86.4486008

-

0.29333

11779

ygaC

0.7205215693

150 nt

3341463

3341612

reverse

elbB,mtgA

-5561

-57.50

-

0.5000

84.6742020

-

0.28000

3454

mlaB,mlaC,mlaD,mlaE,mlaF

0.7152099609

150 nt

1950122

1950271

reverse

torY,torZ

-2401

-56.44

-

0.5200

85.7341003

-

0.24666

1654

aspS

0.7131192684

150 nt

3059434

3059583

reverse

ygfI

-4786

-56.20

-

0.4933

84.9087982

-

0.27333

1879

yqfE

0.7122740149

150 nt

2530006

2530155

reverse

pdxK

-4323

-58.20

-

0.5000

84.2724991

-

0.25333

829

zipA

0.7098694444

150 nt

757742

757891

reverse

mngR

-6555

-57.90

-

0.4867

83.9291000

-

0.31333

4129

gltA

0.7092900276

150 nt

4452551

4452700

reverse

fbp

-4

-56.90

-

0.5200

85.3691025

-

0.29333

4954

ppa

0.7049999237

150 nt

3584997

3585146

reverse

ugpA,ugpB,ugpC,ugpE,ugpQ

-317

-59.30

-

0.5133

84.1650009

-

0.32000

229

ggt

0.7046704292

150 nt

3459796

3459945

reverse

bfd,bfr

-4396

-59.60

-

0.5200

84.1896973

-

0.23333

6454

gspA,gspB

0.7009978294

150 nt

3395856

3396005

reverse

mreB,mreC,mreD

-474

-56.20

-

0.5000

84.8627014

-

0.25333

1654

yhdP

0.6998521686

150 nt

2321601

2321750

reverse

yfaA,yfaP,yfaQ,yfaS_1,yfaS_2,yfaT

-3709

-61.10

-

0.5333

83.9000015

-

0.24000

3829

rcsC

0.6947578788

150 nt

4169919

4170068

reverse

coaA

-2101

-56.20

-

0.4933

84.4697037

-

0.26000

8704

trmA

0.6920779943

150 nt

4029970

4030119

reverse

mobA,mobB

-8880

-57.80

-

0.5133

84.4968033

-

0.30000

1054

fadA,fadB

0.6919249892

150 nt

2366493

2366642

reverse

pmrD

-4722

-56.00

-

0.5200

85.1843033

-

0.28000

2929

ais

0.6792802215

150 nt

1850076

1850225

reverse

ydjE

-490

-57.40

-

0.5267

84.6417999

-

0.27333

3454

selD,topB,ydjA

0.6695327163

150 nt

3246739

3246888

reverse

yhaJ

-4522

-56.00

-

0.4867

83.8035965

-

0.26000

4054

uxaA,uxaC

0.6671289802

150 nt

3883703

3883852

reverse

purP

-9513

-56.60

-

0.5200

84.5989990

-

0.28000

2029

dnaA,dnaN,recF

0.6626442075

150 nt

4395241

4395390

reverse

yjfN

-18720

-56.20

-

0.5267

84.9857025

-

0.29333

3229

queG

0.6626062393

150 nt

2664571

2664720

reverse

hcaT

-79

-56.20

-

0.5267

84.7899017

-

0.24666

3304

trmJ

0.6529098153

150 nt

2975533

2975682

reverse

lysA

-47

-57.20

-

0.5333

84.5955963

-

0.24666

1204

omrB

0.6521243453

150 nt

796718

796867

reverse

ybhA

-39

-58.40

-

0.5333

83.8794022

-

0.26666

2929

modE,modF

0.6405209899

150 nt

3288088

3288237

reverse

rsmI

-2330

-55.90

-

0.5067

83.9057007

-

0.28000

11479

agaR

0.6363855004

150 nt

3211791

3211940

reverse

mug

-1119

-56.10

-

0.5200

84.1829987

-

0.25333

3304

tsaD

0.6315983534

150 nt

2161503

2161652

reverse

ogrK

-3744

-55.70

-

0.5333

84.3114014

-

0.29333

10429

yegK,yegL

0.6064385772

150 nt

3284413

3284562

reverse

rsmI

-6005

-55.60

-

0.5333

84.2777023

-

0.25333

7804

agaR

0.6025381684

  1. Significant hits of the forward and reverse strands of the E. coli intergenic regions having high RND entropy (p-Val.<0.500), significantly low (p.Val. <0.050), GC and uracil compositions within the range of those for known riboswitches Threshold values and their corresponding p-values have been calculated separately for each genome-wide test. 50 nt overlap used for 100 nt scan (100090 segments). 175 nt overlap used for 150 nt scan (66414 segments). Distance from upstream and downstream operons are the distance from the center of the hit to the stop and start codons of upstream and downstream operons, respectively. Probability denotes the multinomial regression likelihood of being a riboswitch under the LMFEGCRND model. Positions are according to gbU00096.2 version of E. coli and not gbU00096.3 version. Negative values indicate distance to upstream operon. Columns Upsream/Downstream Operon show gene ID within the operon.
  2. 1Table 30: Complete list of genes in this operon is gspC,gspD,gspE,gspF,gspG,gspH,gspI,gspJ,gspK,gspL,gspM,gspO.