Figure 1From: Aro: a machine learning approach to identifying single molecules and estimating classification error in fluorescence microscopy images GUIs in Aro. A. The training GUI. The left plot is a 16 × 16 square of pixels from the image on the right with local maxima (candidate spots) marked in blue. The user has the option to designate the maxima as signal, noise and add them to the training set or to skip them. B. The reviewing GUI. The left plot is a grid containing each identified local maximum ranked by its vote. Blue outlines mark signal spots; yellow boxes mark noise spots. The user has the option to correct classifications and retrain the classifier. The image on the right shows the context for the spot currently in focus (red outline).Back to article page