Figure 2
From: Pydna: a simulation and documentation tool for DNA assembly strategies using python
Outline of the cloning strategy described for the construction of YEp24PGK-XK. The Saccharomyces cerevisiae XKS1 gene was amplified by PCR from chromosomal DNA using primers 1 and 3. The PCR product was digested with BamHI and the flanking stuffer fragments removed. The vector YEp24_PGK was digested with BglII and the linear vector and the digested PCR product were ligated together using T4 DNA ligase resulting in the YEp24PGK_XK vector. The supplementary data contains a pydna script that will automatically assemble the YEp24PGK_XK vector.