Figure 3
From: Pydna: a simulation and documentation tool for DNA assembly strategies using python
Outline of the cloning strategy described for the construction of pGUP1. The Saccharomyces cerevisiae GUP1 gene was amplified with primers GUP1rec1sens (green) and GUP1rec2AS (red). The plasmid vector pGREG505 was digested with SalI that cuts the vector in two locations flanking the HIS3 marker. The PCR product is joined by in-vivo homologous recombination to the linear vector fragment aided by short stretches of homology introduced in the PCR process.