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Table 2 Relative amount of GFP between high and low expression system at different stage of cell growth

From: Comparative proteomics: assessment of biological variability and dataset comparability

Samples Comparison a R TS Internal standard b Relative amount of GFP
     LC-MS/MS c FL d
High-1 vs Low-1 1A/3A 1.34 −1.43 ± 0.44 2.49 ± 0.57 2.86 ± 0.42
  1B/3A 1.12 −1.03 ± 0.32 2.66 ± 0.71  
  1C/3A 1.29 −1.07 ± 0.38 2.51 ± 0.75  
  1A/3B 1.05 −1.23 ± 0.66 3.88 ± 1.69  
  1B/3B 1.14 1.23 ± 0.53 2.12 ± 0.66  
  1C/3B 1.65 1.22 ± 0.77 2.06 ± 0.94  
  1A/3C 1.88 −1.44 ± 0.87 7.09 ± 3.17  
  1B/3C 1.57 −1.01 ± 0.51 3.89 ± 1.23  
  1C/3C 1.09 1.35 ± 0.44 3.48 ± 0.59  
High-2 vs Low-2 2A/4A 1.18 −1.09 ± 0.27 3.27 ± 0.70 4.00 ± 0.62
  2B/4A 1.14 −1.12 ± 0.37 3.66 ± 1.08  
  2C/4A 1.14 −1.14 ± 0.34 4.00 ± 1.09  
  2A/4B 1.04 −1.04 ± 0.19 3.87 ± 0.61  
  2B/4B 1.01 −1.06 ± 0.20 4.28 ± 0.76  
  2C/4B 1.00 −1.09 ± 0.27 4.73 ± 1.10  
  2A/4C 1.05 −1.06 ± 0.23 3.80 ± 0.79  
  2B/4C 1.02 −1.08 ± 0.30 4.24 ± 1.06  
  2C/4C 1.02 −1.05 ± 0.19 4.58 ± 0.89  
  1. aNumber indicates the group of sample and A, B, and C indicates the biological replicates as described in Table 1.
  2. bInternal standard is the average relative amount of internal standards between two biological replicates.
  3. cThe relative amount of GFP between two biological replicates of samples calculated by the spectral counting method with the use of internal standards.
  4. dFluorescence was measured in triplicate from separate biological replicates.