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Table 3 Protein scoring system for the protein determination in replicates

From: Comparative proteomics: assessment of biological variability and dataset comparability

a Pep uniq

 

b -Log (e)

Score ( S )

≥2

AND

≥10

3

≥2

OR

≥10

2

=1

AND

2 ≤ −Log(e) < 10

1

  1. aPepuniq is the number of unique peptide used to the protein identification.
  2. b-Log (e) is the expectation value of protein identification by X!Tandem.
  3. Identification score of protein k (ID_S(P k )) was calculated as a sum of each scores obtained from each replicates. When n = 3 (triplicate), protein with ID_S(P k ) ≥ 3 was considered present in a sample.
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BMC Bioinformatics

ISSN: 1471-2105

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