|STEP 1||Perform maximum intensity projection of the detected 3D synapse object.|
|STEP 2||Obtain the contour points of the synapse cluster.|
|STEP 3||Calculate the concavity score of the contour points based on Eq. (2).|
|STEP 4||Choose a detected marker (denoted M1) from the markers pool P, whose sum distance from the rest of the markers is maximum.|
Split M1 from the rest of the markers.|
Start from the highest concavity point, A, look for a point B so that segment AB splits marker M1 from the rest N-1 markers. If no such segment was found, move to the contour point with next highest concavity and repeat until a good split is found. When multiple segments AB were found, the optimal pair that maximizes Eq. (1) us used.
|STEP 6||Assign new identity to voxels of the newly split synapse for quantification statistics.|
|STEP 7||REPEAT STEP 4, until P only has one marker left.|