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Fig. 4 | BMC Bioinformatics

Fig. 4

From: Robust and automated three-dimensional segmentation of densely packed cell nuclei in different biological specimens with Lines-of-Sight decomposition

Fig. 4

Cell nuclei in different biological specimens acquired with different imaging techniques. (a) Raw data showing single slices of three-dimensional stacks of three test datasets. From top to bottom: CD1 mouse embryo labelled with DAPI (scale bar 10 μm). In total 235 two-dimensional slices. Recorded with a Zeiss confocal microscope (LSM 780, AxioObserver), objective lens EC Plan-Neofluar 40x/1.30 Oil Ph3. Cellular spheroid of human breast cancer cells (T47D) labelled with H2B:GFP (scale bar 10 μm). In total 296 two-dimensional slices. Recorded with a digital scanned laser light sheet-based fluorescence microscope (DSLM), objective lens Epiplan-Neofluar 10x/0.3 NA. Large cellular spheroid of BxPC3 human pancreatic cancer cells labelled with DRAQ5 (scale bar 20 μm). In total 465 two-dimensional slices. Recorded with a selective plane illumination microscope (SPIM), objective lens CZ 40x/0.8 NA water dipping. (b) Three-dimensional image reconstruction of respective stacks of raw images

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