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Fig. 5 | BMC Bioinformatics

Fig. 5

From: Robust and automated three-dimensional segmentation of densely packed cell nuclei in different biological specimens with Lines-of-Sight decomposition

Fig. 5

Comparison of LoS decomposition of the three test datasets with FARSight, 3D Watershed and ilastik. Results for (a) the mouse embryo dataset, (b) the breast cancer spheroid dataset, and (c) the pancreatic cancer spheroid dataset. In each case, the top row shows the maximum projection of the raw three-dimensional data and the segmentation output generated by LoS, FARsight, 3D Watershed and ilastik. Each segmented object is assigned a different color. The bottom row shows a two-dimensional section of the dataset (slice number for dataset (a): 123, (b): 146, and (c): 292) and the corresponding sections of the segmentations. Scale bars: (a) 10 μm, (b) 20 μm, (c) 20 μm

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BMC Bioinformatics

ISSN: 1471-2105

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