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Fig. 9 | BMC Bioinformatics

Fig. 9

From: Robust and automated three-dimensional segmentation of densely packed cell nuclei in different biological specimens with Lines-of-Sight decomposition

Fig. 9

Quantitative comparison of nuclei volumes measured in segmentation results of LoS, FARSight, 3D Watershed and ilastik. (a) Mouse embryo, (b) breast cancer spheroid and (c) pancreatic cancer spheroid. Data is visualized as box-and-whisker plots: the white lines within the boxes describe the respective mean, the error bars show variability outside the upper and lower quartiles and the dots represent outliers. The gray vertical line describes the mean of the respective ground truth nuclear volume that was manually determined for a subset of nuclei in all test datasets. Note: for better visualization extreme outliers from the segmentation results of ilastik were removed (one for (a), one for (b) and three for (c))

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BMC Bioinformatics

ISSN: 1471-2105

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