Fig. 4

The dual-strand barplot. Detailed Legend: The barplot from the AllelicImbalance package shows the number of reads aligning to each allele and strand for one SNP. Upward bars indicate the (+) strand, and downward bars indicate the (−) strand. The numbers under the bars are the p-values from testing whether a difference in allele expression is due to chance. Because the data are strand-specific, nearly all reads over this SNP are mapped to one strand, consistent with the location of the investigated genes. All samples in this figure are from liver. a Of the heterozygote individuals 5–7, 5 and 7 show no AI, whereas individual 6 shows significant AI. But, the plot also shows expression off of the opposite strand, which might comprise antisense transcripts. b Individuals 3, 4, and 5 show AI. c Individuals 1, 2, and 6 show strong AI. d Individuals 1 and 4 show AI, whereas subjects 5, 6, 7, and 8 show no AI. See Additional file 1 and 2 for barplots using different aligners