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Fig. 4 | BMC Bioinformatics

Fig. 4

From: WormGUIDES: an interactive single cell developmental atlas and tool for collaborative multidimensional data exploration

Fig. 4

Toward integration of neural morphology into WormGUIDES. a. Schematic of the neuron shape characterization strategy. Multiple strains with different subsets of labeled neurons are lineaged and segmented. Nuclear positions are used to align data to a single reference coordinate system. b. Key to lim-4 expressing cells identities. c. A time lapse 3D reconstruction of pairs of left right symmetric neurons expressing lim-4 imaged using a diSPIM system. Close, difficult to resolve clusters of cells are segmented as a single object. Nuclei are rendered as small gray spheres. Cell bodies are colored to show left right symmetry. A series of images of the reconstruction spanning 40 min is shown. Time 0 is an arbitrary point where lim-4 expression is clearly visible; Time 40 is approximately 10 min before twitching begins. Cell lineage and cell shape are semi-automatically segmented and tracked

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