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Fig. 2 | BMC Bioinformatics

Fig. 2

From: QoRTs: a comprehensive toolset for quality control and data processing of RNA-Seq experiments

Fig. 2

A small selection of the QC plots offered by QoRTs. This series includes 12 samples, each consisting of 6 technical replicates (for a total of 72 bam files), with 4 different biological conditions (3 samples per condition). In all nine plots, replicates are colored and differentiated by biological group. In the line plots (c,d,e, and f) the samples are simply colored by biological group. In other plots (a and g), replicates are differentiated by character, color, and horizontal offset. This differentiation allows easy identification of both outliers and systematic biases or errors associated with the biological condition. Such systematic errors are of particular importance as they could potentially drive false associations. A full description of each plot and its interpretation can be found in the supplementary materials

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