ImmunExplorer (IMEX): a software framework for diversity and clonality analyses of immunoglobulins and T cell receptors on the basis of IMGT/HighV-QUEST preprocessed NGS data

Table 1 Basic analysis in IMEX of the IMGT/HighV-QUEST sequence alignments for the TR using primer set 1 and 2 of proband p78690

Proband: p78690	TRB primer set 1			TRB primer set 2			TRB primer set 2:	TRB primer set 2:
							CD4-/CD8+	CD4+/CD8-
	T1	T2	T3	T1	T2	T3	T3	T3
Productive	16,707	104,346	91,840	77,380	77,997	148,676	224,397	170,696
Unproductive	4018	22,534	18,739	9382	16,066	27,577	42,477	32,884
Unknown	4461	2639	1994	1424	67	1102	1403	1167
No result	11,989	10,879	9068	14,268	1162	14,694	11,498	8135
Total number of sequences	37,175	140,398	121,641	102,454	95,292	192,049	279,775	212,882

For each TR primer set (T cell receptor β chain amplification based on Biomed-2 primer sets) we have determined the total number of productive, unproductive, unknown and no result sequences. For primer set 2 at time point 3 we prepared an additional basic analysis for CD4-/CD8+ and CD4+/CD8- sorted cells

ISSN: 1471-2105