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Table 1 Primer sets used for amplification and sequencing of Coxsackievirus B2

From: Phylodynamic reconstruction of the spatiotemporal transmission and demographic history of coxsackievirus B2

Gene

Primera

Sequence

Positionb

Reference

VP3

2400F

GCTTTGTGTCTGCMTGYAATGA

2372-2393

CDC-TWc

VP1

222R

CICCIGGIGGIAYRWACAT

2909-2891

[20]

VP1

292F

MIGCIGYIGARACNGG

2550-2565

[20]

2A

011R

GCICCIGAYTGITGICCRAA

3327-3308

[20]

3Dpol

PY-03F

GTYACMTATGTGAARGATG

6383-6398

This study

3Dpol

PY-04R

CTTCATTGGCATTACTGGATG

7104-7085

This study

  1. aF: Forward primer, R: reverse primer
  2. bNumbering system used for the Coxsackievirus B2 strain (Accession No. AF085363)
  3. cPrimer designed by the Centers for Disease Control, Taiwan