Skip to main content
Fig. 1 | BMC Bioinformatics

Fig. 1

From: Improving RNA-Seq expression estimation by modeling isoform- and exon-specific read sequencing rate

Fig. 1

An example of gene structure with junction exons and the additional noise isoform. This example gene originally contains four exons, e1, e2, e3 and e4, and two annotated splice variants, isoform1 and isoform2, as shown in the dotted rectangle. According to the constitution of the two splice variants, three junction exons are constructed, e1-e3, e1-e2 and e2-e4. We also include an additional splice variant containing all annotated and junction exons as shown on the bottom of the figure. On the top of the figure, we show two example reads, read1 and read2, among which read1 was aligned to the junction of e1 and e2, and read2 was aligned to e3. When counting the fragment number for each exon, we add one to the fragment counts of e1-e2 and e3 respectively

Back to article page