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Table 3 Comparison results of various methods for SELC dataset on calculated log 2 fold change of gene expression

From: Improving RNA-Seq expression estimation by modeling isoform- and exon-specific read sequencing rate

Comparison

Gene

qRT-PCR

Cufflinks

RSEM

MMSEQ

NLDMseq

S vs. NS

S100A8

+1.15

+1.13(0.02)

+2.72(1.38)

+3.20(1.78)

+1.65(0.44)

 

S100A9

+0.83

+1.69(1.03)

+1.79(1.16)

+1.86(1.24)

+1.75(1.10)

 

CYP4F2

+1.27

+4.12(2.24)

+3.59(1.82)

+9.51(6.49)

+2.09(0.64)

C vs. NC

CCL20

+4.01

+6.00(0.50)

+4.66(0.16)

+5.32(0.32)

+7.08(0.76)

 

IL8

+1.50

+1.51(0.01)

+1.21(0.19)

+1.67(0.11)

+1.05(0.30)

 

SCGB3A1

+0.51

+1.65(2.23)

+1.76(2.45)

+1.85(2.63)

+1.82(2.57)

 

SCGB1A1

−0.48

+1.37(3.89)

+1.56(4.26)

+1.02(3.13)

+1.62(4.39)

Average AER

NA

1.42

1.63

2.24

1.46

  1. The symbol “ +” stands for up-regulation and “ −” for down-regulation. Numbers in the brackets stand for absolute error rates (AER) compared with qRT-PCR results. AER is calculated by |(re)/r|, where |·| stands for absolute, and r and e represent qRT-PCR and RNA-seq measurements, respectively. The last line shows the average AER for each method