Fig. 4

Multiple sequence alignment of representative members of the GmrSD family. The alignment contains double-domain protein sequences (indicated as GmrSD), GmrS-domain only (GmrS) and GmrD-domain only (GmrD). Domain ranges are indicated above the alignment. Genus and species name abbreviations used: Baccer–Bacillus cereus, Bacfra–Bacteroides fragilis, Censym–Cenarchaeum symbiosum, Chlvar–Chlorella variabilis, Clobot–Clostridium botulinum, Cloper–Clostridium perfringens, Cocsub–Coccomyxa subellipsoidea, Corglu–Corynebacterium glutamicum, Esccol–Escherichia coli, Haeinf–Haemophilus influenzae, Halwal–Haloquadratum walsbyi, Helpyl–Helicobacter pylori, Lismon–Listeria monocytogenes, Metsmi–Methanobrevibacter smithii, Myccap–Mycoplasma capricolum, Mycfer–Mycoplasma fermentans, Mycmob–Mycoplasma mobile, Mycmyc–Mycoplasma mycoides, Pseaer–Pseudomonas aeruginosa, Salent–Salmonella enterica, Staepi–Staphylococcus epidermidis, Stahae–Staphylococcus haemolyticus, Strcoe–Streptomyces coelicolor, Strmut–Streptococcus mutans, Strpne–Streptococcus pneumoniae, Thaoce–Thalassiosira oceanica, Vibcho–Vibrio cholerae. Kingdom information: (A) – Archaea, (B) – Bacteria, (E) – Eukaryota. UT, CT – GmrSD UT and GmrSD CT enzymes [11, 74]. Residues conserved in > 95 % sequences are marked by “+”. The color-shading in this figure represents sequence conservation in the selected subset of proteins only. The complete alignment of 1094 sequences is available as Additional file 1. Consensus secondary structure is indicated above the alignment as tubes (helices) and arrows (strands) for the E. coli GmrSD UT protein