Evaluation of shotgun metagenomics sequence classification methods using in silico and in vitro simulated communities

Table 1 Microbes used in the 2 simulated mock communities

MetaSimHC^a			Freshwater^b (FW) in silico and in vitro
Genus	Species	Strain	Genus	Species	Strain
Agrobacterium	tumefaciens	C58	Bacillus	amyloliquefaciens	FZB42
Anabaena	variabilis	ATCC 29413	Bacillus	cereus	ATCC 14579
Archaeoglobus	fulgidus	DSM 4304	Burkholderia	cenocepacia	J2315
Bdellovibrio	bacteriovorus	HD100	Escherichia	coli	K-12
Campylobacter	jejuni	81–176	Frankia	sp.	CcI3
Clostridium	acetobutylicum	ATCC 824	Micrococcus	luteus	NCTC 2665
Lactococcus	lactis	SK11	Pseudomonas	aeruginosa	PAO1
Nitrosomonas	europaea	ATCC 19718	Pseudomonas	aeruginosa	UCBPP-PA14
Pseudomonas	aeruginosa	PA7	Pseudomonas	fluorescens	Pf-5
Streptomyces	coelicolor	A3(2)	Pseudomonas	putida	KT2440
Sulfolobus	tokodaii	str. 7	Rhodobacter	capsulatus	SB 1003
			Streptomyces	coelicolor	A3(2)

^aMetaSimHC is a test dataset of 11 diverse microbial genomes covering several phyla of Bacteria and Archaea proposed in [21]
^bFreshwater (FW) is a set of bacterial genomes found in previous freshwater metagenomics studies (see Methods)

ISSN: 1471-2105