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Table 1 Overview of the samples used for validation

From: Fully automated registration of vibrational microspectroscopic images in histologically stained tissue sections

Id

Type

Size (pixels)

 

Number and sizes of spectral images

S1

Colon

(1915×994)

7

(336×128), (240×128), (240×128), (240×128), (240×128), (384×96), (288×160)

S2

Colon

(2402×1872)

3

(240×160), (192×128), (288×128)

S3

Colon

(1203×987)

6

(288×128), (288×160), (192×128), (288×128), (192×32), (192×32)

S4

Colon

(2666×1720)

5

(288×128), (336×128), (96×488), (288×128), (96×488)

S5

Bladder

(319×257)

1

(192×192)

S6

Bladder

(3000×3615)

1

(2816×2432)(whole slide)

S7

Lung

(5334×4000)

1

(3840×3712)(whole slide)

S8

Colon

(3946×4200)

1

(2304×2688)(whole slide)

S9

Colon

(4500×3551)

1

(4096×3804)(whole slide)

S10–S15

Lung

(1392×886)– (2491×2227)

2–5

(212×354)– (618×237)

S16–S28

Bladder

(1639×947)– (2985×1676)

3

(212×354)– (618×237)

TMA

Colon

 

56 cores

∼(375×375)(approx. size of one spot)

C1

Lung

(825×825)

1

(1024×1024)

C2

Lung

(1473×1845)

1

(1024×1024)

  1. Spectral images for samples S1–S28 and TMA are available as FTIR images, while spectral images for samples C1 and C2 are CARS microscopic images. For samples S1–S28 and TMA, the resolution of the H&E images has been adjusted to approximately match the resolution of the corresponding FTIR images. Note that for the CARS samples C1 and C2, the spatial resolution of the CARS images is higher than the resolution of the H&E images, so that the CARS subregions involve significantly more pixels than the H&E images, which cover a much larger area. This difference in resolution for CARS is resolved by taking into account scaling during registration. Full details on samples S10–S28 are provided in Additional file 1: Table S1
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BMC Bioinformatics

ISSN: 1471-2105

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