Fig. 4

Four alternative ORF sequences. The cDNA with artificial frame-shift (FS) is shown along with four possible ORFs, all of which are aligned to the known CDS sequence. The first ORF has accurately corrected the frame-shift, producing an in-frame result. The second ORF has not made any changes to the input sequence and so is not in frame. The third ORF excludes the frame-shift position, but the resulting sequence is in-frame. The final ORF excludes the frame-shift position, but a frame-shift correction has still been made. Finally we show which of the ORFs are accurate, and then which of the sequences with an attempted FSC (ORFs 1 and 4) are accurate. Alternating codons are shown in different shades of blue