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Fig. 4 | BMC Bioinformatics

Fig. 4

From: Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images

Fig. 4

Morphological features of G1, S, G2, and M phase of the cell cycle. a Thresholding of raw cell images via Otsu's method to partition DNA signal into foreground and background pixels. b Spatial extent of DNA fluorescence, assayed by counting the number of foreground pixels in thresholded images. On average, G1 is smaller than S is smaller than G2. See Additional file 2: Tables S9, S10 for statistical comparisons. c Spottiness of DNA morphology, assayed by counting the number of connected components in thresholded images. On average, G1 and G2 phase cells have more connected components than S phase cells, which have more connected components than M phase cells. See Additional file 2: Tables S7, S8 for statistical comparisons

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BMC Bioinformatics

ISSN: 1471-2105

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