Table 1 ECBC Pipeline Analysis on Non-host Reads of Samples Containing B. anthracis, F. tularensis, Y. pestis, B. pseudomallei, and B. mallei. Unknown samples were created, sequenced on 454, Ion Torrent, and Illumina platforms and processed (methods). Datasets were then analyzed using the ECBC pathogen detection pipeline. Table shading represents the positive and correct identification of the organism listed. Unshaded cells represent the lack of single-read identification matching to the pathogens spiked
454 | Ion Torrent | Illumina | |||||
|---|---|---|---|---|---|---|---|
File size: 2.5gb | File size:1.62gb | File size: 5gb paired-read files | |||||
Pipeline runtime: 35m12s | Pipeline runtime: 29m18s | Pipeline runtime: 4hr11m | |||||
Agent | Spiked Amounts | Taxonomy Assignment ID | Iterative Assembly ID | Taxonomy Assignment ID | Iterative Assembly ID | Taxonomy Assignment ID | Iterative Assembly ID |
B. anthracis | 1x106 CFU |
|
|
|
|
|
|
F. tularensis | 1x105 CFU |
|
|
|
|
|
|
Y. pestis | 1x104 CFU |
|
|
|
|
|
|
B. pseudomallei | 1x103 CFU |
|
| ||||
B. mallei | 1x102 CFU |
| |||||
