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Table 3 Iterative Analysis on Non-host Reads of Sample 712, 806, 808, 819, 820, 1500, and 1501. Collected known samples (Table 2) were analyzed using the ECBC iterative analysis pipeline for pathogen detection. De novo assembled contigs are used to generate nearest neighbors, then the nearest neighbors are used to map reads and generate consensus contigs from the mapped reads (Fig. 2). Upon completion, a new iteration begins using reads not mapped to the nearest neighbor. The cycle completes after no further reads exist for contig building or there are no matches reported

From: Pathosphere.org: pathogen detection and characterization through a web-based, open source informatics platform

Sample Iteration Reads de novo Contigs Nearest Neighbor Reported Contigs Generated from Nearest Neighbor Read Mapping
712 1 759 3 Lujo virus segment S glycoprotein precursor and nucleocapsid protein genes, complete cds 2
2 90 1 Lujo virus segment L multifunctional matrix-like protein and large RNA-dependent RNA polymerase genes, complete cds 1
806 1 4752 0 - -
808 1 4479 4 Lujo virus segment L multifunctional matrix-like protein and large RNA-dependent RNA polymerase genes, complete cds 3
2 1527 1 Lujo virus segment S glycoprotein precursor and nucleocapsid protein genes, complete cds 1
3 139 0 - -
819 1 12450 12 Zaria bat coronavirus strain ZBCoV, partial genome 1
2 8247 9 - no db hits
820 1 14790 31 Rhinolophus ferrumequinum clone VMRC7-267P18, complete sequence 1
2 10222 37 Zaria bat coronavirus strain ZBCoV, partial genome 1
3 9704 33 - no db hits
1500 1 884863 3993 Middle East respiratory syndrome coronavirus complete genome 1
2 26795 37 Actinobacillus suis H91-0380 complete genome 1
3 26643 36 Middle East respiratory syndrome coronavirus Isolate Qatar4 complete genome 12
1501 1 828116 3967 Middle East respiratory syndrome coronavirus complete genome 1
2 26937 33 Actinobacillus suis H91-0380 complete genome 17
3 26761 34 PREDICTED: Equus caballus uncharacterized LOC102148405 (LOC102148405) 1