Skip to main content
Fig. 4 | BMC Bioinformatics

Fig. 4

From: Unsupervised image segmentation for microarray spots with irregular contours and inner holes

Fig. 4

The geometrical features of each spot are approximated by a foreground ellipse E F (broken line) and a background ellipse E B (solid line). The approximation is shown for two groups of spots, i.e., two sub-images of image AT20391 (dye Cy3) depicted in figure panels a and b respectively. Spots of low intensity and non-spherical profiles, as well as artifacts of high background signal, make the identification and description of foreground and background area non-trivial. The ellipses show a rather high diversity in size and form but give a reasonable initial approximation of the foreground and background area of each spot

Back to article page