Fig. 6
From: IPLaminator: an ImageJ plugin for automated binning and quantification of retinal lamination
Calbindin as an alternative to ChAT for strata demarcation. a Section of wild type retina stained with antibodies to Syt2, ChAT and calbindin. b Gray scale intensity profiles for Syt2, ChAT and Calbindin. c Use of ChAT based layer separation on three channels. d Use Calbindin based layer separation on three channels. We see very marginal difference between the two mainly the middle retina layers. Scale bar (in A) =105.8 μm