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Table 1 Obtained and validated SNPs per parameter set. Of the six tested parameter combinations, the lowest false positive rate was retrieved with the default values: 15 Minimum coverage and 70 Minimum average quality

From: 4Pipe4 – A 454 data analysis pipeline for SNP detection in datasets with no reference sequence or strain information

 

Parameters used (Min. Average Quality)

10|60

10|70

15|60

15|70 (Default)

10|75

20|70

Total SNPs retrieved

234

169

155

114

107

89

Confirmed SNPs

97

86

88

82

69

57

False Positive rate (%)

58.55

49.11

43.23

28.07

35.51

35.96