Fig. 3

Evaluating TIN metric using transcript level RNA fragment size. The average RNA fragment size (y-axis) of a particular transcript was estimated from all the read pairs that uniquely mapped to the transcript (see Methods). a Correlation between TIN score and transcript level RNA fragment size. A single GBM sample (SRR873822; RIN = 10) was used to produce the figure. Each dot represents 50 transcripts. Red curve indicates the locally weighted polynomial regression curve. b Locally weighted polynomial regression curves for all GBM RNA-seq samples