Fig. 5
From: Trimming of sequence reads alters RNA-Seq gene expression estimates
Isoform and gene expression levels after length-filtering. a The distribution of input read lengths before (blue) and after (red) trimming. b Comparison of the expression estimates of isoforms and genes between the untrimmed data set and the trimmed data sets with minimum read lengths required. L# specifies the minimum read length, in bases, required for inclusion. Red dots represent statistically significant differential expression between data sets. c The number of significantly biased isoforms and (d) genes at a range of minimum read lengths