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Fig. 9 | BMC Bioinformatics

Fig. 9

From: Illumina error profiles: resolving fine-scale variation in metagenomic sequencing data

Fig. 9

Overview of Illumina sequencing using reversible dye-terminating ddNTPs: The template DNA sequences including the primers are first immobilised on a solid surface. During each cycle the polymerase incorporates one reversible dye-terminator base (ddNTP). The synthesis is temporarily paused and the dye is excited with a laser to identify the incorporated nucleotide. All remaining ddNTPs are washed off and the fluorescent tag and 3’-O blocking group is removed. This is followed by another washing step before the DNA polymerase recommences

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