Fig. 4

The model of the Pseudomonas 16S rRNA gene fragment integration into CEACAM5 of participant A is presented. The structure of the Pseudomonas 16S rRNA gene fragment, the first exon CEACAM5, and the paired reads supporting the integration of the rRNA gene fragment into CEACAM5 are illustrated (a). Calculations using the JSD (b) and AD (c) support that there are 26 bp between the Pseudomonas 16S rRNA & CEACAM5 fragments and that the integration is in 48–73 bp of CEACAM5 (f). The CEACAM5 sequence upstream of the bacterial 16S rRNA fragment is for illustrative purposes only (f). The insert-size for each paired-end read is color-coded with lighter colors being closer to the median insert-size, red designating those insert-sizes larger than the median, and green for insert-sizes less than the median as further clarified in Additional file 7: Figure S7. The optimum model for the structure of the integration (f) is compared to alternative distances (x) between the two fragments in (d-h), where an additional 1.0, 0.5, −0.5, & -1.0 median absolute deviations (20 bp) are placed between the two fragments represented by the gray region. The actual DNA sequence of the gray region is unknown