
LifeTech method


0.1/0.1 vs pure

0.01/0.01 vs pure

0.01/0.01 vs 0.1/0.1

0.50

27.6

26.8

26.3

0.75

28.9

28.4

28.4

1.00

29.1

29.0

29.2


qpcR Method


0.1/0.1 vs pure

0.01/0.01 vs pure

0.01/0.01 vs 0.1/0.1

0.50

26.9

25.8

25.3

0.75

29.2

28.3

28.6

1.00

30.1

29.3

29.9

 Here we report three potential limits of detection based on the three assessments (0.1/0.1 vs pure, 0.01/0.01 vs pure, and 0.01/0.01 vs 0.1/0.1). Limits of detection are reported separately for the Life Technologies (LifeTech) and qpcR algorithms. Columns correspond to the different assessments, and rows correspond to the median difference between the observed and expected values. The values in the matrix are the expected expression values such that the median absolute difference for all larger expected expression values is approximately equal to the threshold for that row. For example, consider the Life Technologies method, if we focus on the 0.1/0.1 vs 0.01/0.01 comparison (column 3) and set a median average difference threshold of less than 1.00 (row 3), our estimate of the limit of detection is approximately 29.2