Fig. 1

Overview of experimental barcoding technology and barcode quantification. In brief, progenitor cells isolated from organs (e.g. bone marrow) are labeled with unique, heritable barcodes (represented by differently coloured cells). Barcoded cells are injected into animals, after which cellular proliferation, differentiation and death occurs. Different cell types are then harvested, DNA is extracted, and the resulting samples are split into technical replicates. These undergo PCR amplification and deep sequencing, resulting in a table with the number of reads for each barcode in each sample