| Barbarini et al. [2] | Pythia-design |
---|
| Binder | Nonbinder | Binder | Nonbinder |
---|
Bound (1:100) | 261 | 25 | 387 | 117 |
Not bound (1:100) | 139 | 175 | 13 | 83 |
Bound (1:400) | 128 | 7 | 325 | 40 |
Not bound (1:400) | 272 | 193 | 75 | 160 |
Bound (1:1000) | 99 | 3 | 270 | 18 |
Not bound (1:1000) | 301 | 197 | 130 | 182 |
- Six hundred peptides representing 400 binders and 200 non-binders of each of Pythia-design and the method of Barbarini et al. were incubated with IVIg. The confusion matrix below indicates that the peptides selected by Pythia-design bind antibodies with higher affinity than the peptides designed by Barbarini et al.